油菜叶片衰老相关基因的分离──ATP硫化酶cDNA的克隆和序列分析

Isolation of Leaf Senescence-related Genes from Brassica napus:Cloning and Sequence Analysis of cDNA for ATP Sulphurylase

通过筛选缩减ｃＤＮＡ文库中在油菜叶片衰老阶段表达而在绿叶中不表达的ｃＤＮＡ克隆，分离了与油菜叶片衰老有关的基因ＬＳＣ６６。ＤＮＡ序列测定和分析结果表明，ＬＳＣ６６的全长可读框架由１３８０个碱基组成，编码４６０个氨基酸，它的核昔酸序列与拟南芥菜ＡＴＰ硫化酶基因有８６．６７％的同源性，其推导的氨基酸序列与拟南芥菜ＡＴＰ硫化酶的氨基酸序列有９１．７４％的同源性。对ＬＳＣ６６基因产物在植物叶片衰老过程中的作用进行了讨论。

LSC66 gene related to leaf seneseecne in Brassica napus was isolated by screeningthe cDNA clones that are expressed during leaf senescence but not expressed in green leavesin a subtracted cDNA library from Brassica napus. DNA sequence analysis indicated that afull length of the open reading frame of LSC66 is composed of 1380bp and encodes 460amino acid residues, and that its nucleotide sequence and deduced amino acid sequenceshowed 86.67 % and 91.74 % similarities to those of ATP, sulphurylase from A rabidopsisthaliana. The possible roles of LSC66 gene product during plant leaf senescence were alsodiscussed.