当归注射液对氢化可的松诱导的大鼠脾脏细胞凋亡的影响

Effect of Angelica Sinensis injection solution on apoptosis of rat spleen cells induced by hydrocortisone

目的：观察当归注射液对氢化可的松诱导的大鼠脾脏细胞凋亡的影响。方法：以氢化可的松诱导大鼠脾脏细胞凋亡为模型，采用流式细胞仪吖啶橙（ＡＯ） 染色法检测凋亡细胞率。结果：１０７ ｍｏｌＬ 氢化可的松作用大鼠脾脏细胞６ ｈ ，ＤＮＡ 片段率显著增高，达２７－４ ％ ，与对照组（１９－４ ％ ） 相比有极显著意义（ Ｐ＜ ０－０１ ，ｎ ＝ ６） ，加入５ ％ 当归注射液后，凋亡率降至２１－７ ％ ，与对照组无显著差异（ Ｐ＞ ０－０５） 。结论：当归注射液可抑制氢化可的松诱导的大鼠脾脏细胞凋亡。

AIM:To invenstigate the effect of Angelica Sinensis injection solution (ASIS) on the apoptosis of rat spleen cells induced by hydrocortisone.METHODS:Flow cytometer with acridine orange straining method was used to observe the rate of cellular apoptosis and the apoptosis of rat spleen cells was induced by hydrocortisone (HYD).RESULTS:When the cells were incubated with 10 7 mol/L HYD for 6 h, the percentage of DNA fragmentation increased to 27.4% compared to the control group 19.4%( P<0.01,n =6),but it decreased to 21 7% ( P >0 05) when 5% ASIS were added.CONCLUSION:ASIS inhibited apoptosis of rat spleen cells induced by HYD and the inhibition is dose-dependent.