肿瘤坏死因子α对正常大鼠胰岛素敏感性的影响

Effect of tumor necrosis factor on insulin sensitivity in rats

目的：探讨注射肿瘤坏死因子α（ＴＮＦα）对正常大鼠胰岛素敏感性及其相关状态的影响。方法：给正常大鼠注射小剂量ＴＮＦα后，以葡萄糖－胰岛素耐量试验（Ｇ－ＩｎｓＴＴ）检测其胰岛素敏感性的变化，并观察血浆胰高血糖素（ＧＣ）、促肾上腺皮质激素（ＡＣＴＨ）、血脂水平及组织中糖原、三酰甘油（ＴＧ）含量的变化情况。结果：发现ＴＮＦα可致正常大鼠出现高胰岛素血症，Ｇ－ＩｎｓＴＴ的Ｋ值下降，血浆ＧＣ，ＡＣＴＨ和血脂水平升高，肝脏和红色股四头肌糖原含量减少，肝脏ＴＧ含量升高。结论：ＴＮＦα可致正常大鼠发生明显的胰岛素抵抗（ＩＲ），这可能与血中胰岛素拮抗激素水平升高或糖代谢、脂代谢紊乱有关。

Objective: To investigate the effect of TNF on insulin sensitivity and some relative states in normal rats. Methods: The changes of K value in glucoseinsulin tolerance test,the concentration of glucagon and ACTH,the lipids in serum and the contents of glycogen,triglyceride in tissues were tested in normal rats after low dose injection of TNF. Results: The K values in glucoseinsulin tolerance test decreased after TNF injected ,the concentrations of ACTH,GC and serum lipids all increased,and the glycogen contents in liver and red quadriceps decreased,the liver TG depots increased. Conclusion: TNF can induce evident insulin resistance in normal rats, the improvement of plasma insulin antagonistic hormone level and some disorders in sugar or lipid metabolism may be involved in.