银屑病与白介素1受体拮抗剂基因多态性

STUDY OF THE ASSOCIATION BETWEEN PSORISAS AND INTERLEUKIN 1 RECEPTOR ANTAGONIST GENE POLYPHISM

用聚合酶链反应（ＰＣＲ）技术对３８例银屑病患者和８５例正常对照组白介素１受体拮抗剂（ＩＬ－ｌｒａ）基因数目可变的串联重复多态性进行了分析，发现银屑病患者中ＩＬ１ＲＮ＊２等位基因频率明显高于正常对照组（Ｐ＜０．０１）。如将３１例寻常型银屑病分为ＰＡＳＩ＜１５和ＰＡＳＩ≥１５两组，则发现前者ＩＬ１ＲＮ＊２频率与正常对照相比无显著差异（Ｐ＞０．０５），而后者有显著差异（Ｐ＜０．０１）；３例脓疱型中的２例，２例关节型及２例红皮病型银屑病患者均携带ＩＬ１ＲＮ＊２等位基因。提示ＩＬ１ＲＮ＊２可能不直接影响银屑病的易感性，但携带ＩＬ１ＲＮ＊２者患病后可能病情更严重。

Polymerase chain reaction(PCR) was used to analyze the polymorphism of variable number of tandem repeat(VNTR)of interleukin 1 receptor antagonist(IL 1ra)gene in 38 patients with psorisas and 85 normal controls.The results showed that the frequency of IL1RN 2 allele was much higher in patients than that of in controls( P <0.01).Among these patients we found that there was no significant difference in the frequency of IL1RN 2 allele between the group (PASI<15) and the controls( P >0.05),but the frequency of IL1RN 2 was significantly increased in the other(PASI≥15)compared with controls( P <0.01),2 to 3 cases of patients with psorisas pustulosa and 2 cases of patients with psorisas arthropathica and 2 cases of patients psorisas erythroderma had IL1RN 2 allele.The results suggest that IL1RN 2 allele might not directly affect the susceptibility to psorisas,but the might be more severe for those patients with IL1RN 2.