慢性肝病患者血清ICAM-1和炎性细胞因子测定

DETECTION OF SERUM INTERCELLULAR ADHESION MOLECULE 1 AND CTOKINES IN CHRONIC LIVER DISEASES

采用双抗体夹心ＥＬＩＳＡ方法对６４例慢性肝炎患者、４３例肝硬化患者和２８例正常人血清细胞间粘附分子（ＩＣＡＭ－１）、白细胞介素－６（ＩＬ－６）、ＩＬ－８、肿瘤坏死因子α（ＴＮＦ－α）水平进行测定。结果：慢性肝炎及肝硬化患者血清ＩＣＡＭ－１及细胞因子水平均明显高于正常（Ｐ＜０．０１），且病情越重其升高越明显。慢性肝炎重度＞中度＞轻度（Ｐ＜０．０５～０．０１）；肝硬化患者高于慢性肝炎患者；失代偿期肝硬化患者高于代偿期肝炎患者（Ｐ＜０．０５）。提示慢性肝病患者血清ＩＣＡＭ－１及炎症相关性细胞因子水平测定可用于判断病情程度及其预后。

To investigate the changes and the relationship between the development of diseases of serum intercellular adhesion molecule 1(ICAM 1) and some proinflammatory cytokines in chronic hepatitis and cirrhosis, we examined serum ICAM 1, interleukin 6(IL 6), interleukin 8 (IL 8) and tumor necrosis factor α(TNF α) in 64 cases of chronic hepatitis, 43 cases of cirrhosis and 28 normal persons by double antibody sandwish ELISA method. The results showed that serum levels of ICAM 1 and cytokines in patients with chronic hepatitis and cirrhosis were significantly higher than that in normal persons. The more severe the disease, the higher the serum levels. Much higher serum concentrations were tested in patients with cirrhosis. Moreover, serum ICAM 1 and the cytokines in incompensatory cirrhosis were higher than that in compensatory one. It is suggested that the detection of serum ICAM 1 and some proinflammatory cytokines in chronic liver diseases can be used to judge the patient’s condition and prognosis.