二氢槲皮素的提取及抗氧化性研究

Extraction and antioxidaion of dihydroquercetin

采用浸提法,分别用水、丙酮-水作为提取溶剂从落叶松中提取二氢槲皮素,通过L9(34)正交实验优化最佳提取条件;研究不同油脂中二氢槲皮素的抗氧化效果;比较Vc、BHT、EDTA、柠檬酸对二氢槲皮素的抗氧化协同作用。结果表明,以水作为溶剂的最佳提取条件为:提取温度90℃、料液比1∶12(g/mL)、提取时间120min,以丙酮作为溶剂的最佳提取条件是:丙酮浓度60%(V/V)%、料液比1∶14(g/mL)、提取时间4 h,且以丙酮-水为提取剂的索氏提取量高于以水作为提取剂的提取量;猪油和大豆油的最佳抗氧化剂添加量分别是0.04%(m/m%)和0.06%(m/m%);在四种增效剂中Vc的增效作用最强。

Dihydroquercetin was extracted from larch with water and acetone-water as extracting solvent respectively.The optimum extracting condition was obtained through the orthogonal experiment design L9（34）.The antioxidant activity of the dihydro-quercetin in different fats was evaluated.The antioxidant synergy of Vc,BHT,EDTA and citric acid on dihydro quercetin was compared.Results indicated that the optimal conditions of water soaking was as follows： extraction temperature was 90℃,liquid ratio was 1∶12（g/mL）,extraction time was 120 min.The optimal conditions of acetone-water soaking was as follows： the concentration of acetone was 60%（V/V）%,liquid ratio was 1∶14（g/mL）,extraction time was 4 h.The extraction rate of soxhlet extraction by acetone-water was higher than that by water.The suitable amount of lard and soybean were added as extraction agent were 0.04%（m/m）% and 0.06%（m/m）% respectively.The synergized action of Vc was the best in the four synergistic agent.