2009年德格鼠疫自然疫源地检测结果分析

Laboratory Detection of Marmota Himalayana in Natural Focus of Plague in Dege,2009

目的研究德格县喜马拉雅旱獭鼠疫自然疫源地鼠疫流行状况。方法对喜马拉雅旱獭等材料进行细菌分离培养、鼠疫间接血凝试验(IHA)和反间接向血凝试验(R IHA)。结果细菌培养检测自毙喜马拉雅旱獭18份,分离鼠疫菌10株,细菌培养检测活体喜马拉雅57份,分离菌株2株;IHA检测旱獭血清56份、阳性1份,牧犬血清24份、阳性2份;RIHA检测组织悬液18份,阳性9份均为旱獭。结论从喜马拉雅旱獭分离出鼠疫菌,说明四川省德格县2009年正在发生动物鼠疫流行。

Objective To investigate the epidemic of Marmota Himalayana plague in natural focus at Dege county in Sichuan province.Methods The detection of Marmota himalayana was conducted by bacteria isolation and culture,indirect Hemagglutination assay（IHA） and reverse indirect hemagglutination assay（RIHA）.Results Twelve strains of Y.pestis were detected from 75 Marmota himalayana samples.One positive sample was detected with IHA in 56 Marmota himalayana serum samples and 2 positive samples were detected with IHA in 24 Sheepdog serum samples.Nine F1 antigen positive samples were detected with RIHA in 18 Marmota himalayana liver-spleen samples.Conclusion Yersinia pestis was isolated from Marmota himalayana,which indicated that the animal epidemic of plague occurred in Dege county in 2009.