人参皂甙与LAK细胞联合治疗恶性脑胶质瘤的实验研究

EXPERIMENTAL STUDY ON CYTOTOXICITY OF GS AND IL-2 ACTIVATED PBMC ON MALIGNANT GLIOMA

目的：探索提高恶性脑胶质瘤治疗效果的新途径；方法：用人参皂甙（Ｇｉｎｓｅｎｏ－ｓｉｄｅ，ＧＳ）与白细胞介素２（ＩＬ－２）协同诱导人外周血单个核细胞（ＰＢＭＣ）制成ＧＳ－ＬＡＫ细胞，用ＭＴＴ法测定其对恶性脑胶质瘤细胞（ＢＴ３２５）的杀伤活性，并与ＬＡＫ细胞相比较；结果：效应细胞ＧＳ－ＬＡＫ在增殖数量和杀伤恶性脑胶质瘤细胞活性等方面均优于ＬＡＫ细胞，且ＩＬ－２用量减少；结论：采用ＧＳ－ＬＡＫ细胞，可提高效应细胞的数量和活性。

Objective:To investigate a new method for inproving the therapeutic effect on malignant glioma.Method:A new type of killer cells,named GS LAK,was induced by means of costimulating the peripheral ginsenoside(GS)and interleukin-2(IL 2).Compared with control group-LAK cells,the cytotoxicity of GS LAK cells against malignant glioma cells(BT325) was examined with MTT method.Results:It showed that GS LAK cells exhibited some advantages over LAK cells in proliferation,cytotoxicity,as well as the utilizing of IL 2.Conclusions:The application of GS LAK cells might open a new prospect for clinical therapeutic approach to malignant glioma.