摘要
目的研究镉对红鲫的免疫毒性和生殖毒性。方法取红鲫28尾,随机分四组,分对照组(不加氯化镉)、低剂量组(0.2 mg/L)、中剂量组(0.8 mg/L)和高剂量组(1.6 mg/L),用含不同浓度镉的曝气水饲养红鲫鱼,采血进行白细胞计数;计算脾脏及生殖系统脏器系数,用石墨炉原子吸收法检测生殖系统的镉含量。结果 1.镉能导致红鲫脾脏脏器系数显著增加,低镉组白细胞数明显高于其它剂量组(P<0.05),而中、高镉组白细胞数与低镉组相比有下降趋势,但与对照组比较差异无统计学意义,表明镉对脾脏有一定的损伤作用。2.镉在生殖系统富集,表明可能造成红鲫鱼生殖系统的损伤。结论镉为红鲫鱼免疫系统和生殖系统毒性物质。
Objective To explore the effect of cadmium on the immune function and reproductive function in red crucian carp. Methods Twenty-eight Carassius auratus red variety were randomly and averagely divided into 4 groups at different doses of cadmium(0.0 mg/L,0.2 mg/L,0.8 mg/L,and 1.6 mg/L).The blood was collected for taking count of the WBC.The spleen and reproductive system were anatomized for counting the organ coefficients.The content of cadmium in the reproductive system was detected by graphite furnace atomic absorption spectrometry. Results Cadmium in red crucian carp could lead to a significant increase in the spleen coefficient.The number of white corpuscle in the low-dose group was significantly higher than that in other groups(P0.05).The number of white blood cell in the middle-dose and high-dose groups was lower than that in the low-dose group,but no statistically significant difference was found between the control group and the middle-dose group or the high-dose group.It was indicated that cadmium could do harm to spleen.The cadmium could be enriched in reproductive system,which indicated that cadmium might bring about injury in the reproductive system of red crucian carp. Conclusions Cadmium could possess immunotoxicity and reproductive toxicity on Carassius auratus red variety.
出处
《实用预防医学》
CAS
2011年第1期7-8,共2页
Practical Preventive Medicine
基金
湖南省卫生厅资助课题(C2005035)
湖南省教育厅资助课题(05C473)
关键词
镉
红鲫
生殖毒性
免疫毒性
Cadmium
Carassius auratus red variety
Reproductive toxicity
Immunotoxicity