培养环境对两性霉素B抑制白色念珠菌作用的影响

Effect of Culture Environment on Activity of Amphotericin B Against Candida albicans

目的综合考察各种因素(不同的培养基、培养基的pH值、真菌接种浓度等)对两性霉素B(AmB)抑制白色念珠菌作用的影响,为临床合理用药提供理论依据。方法液体稀释法。结果在不同的培养基中,AmB对白色念珠菌的MIC值不同,随着pH值的增高及真菌接种菌液浓度增加,其MIC值增加。结论 AmB对白色念珠菌的MIC值会受到多种因素的影响,所以,进行药物的抗菌活性研究时应制定并严格执行统一的标准。

Objective To investigate the effects of various factors,such as medium type,pH value of the medium,and size of inoculum on the activity of amphotericin B（AmB） against Candida albicans,so as to provide the theoretical evidence for rational use of antibiotics. Method The broth dilution test was used in this study. Results The activity of AmB against Candida albicans was influenced by medium type.The minimum inhibition concentrations（MIC） increased with the increase of pH value and inoculum concentration. Conclusions The study of antifungal activity must establish and strictly implement unified standard.