摘要
通过液体发酵、分离纯化生产高纯度纳豆激酶,制备和鉴定纳豆激酶-HP-β-CD包合物,并考察包合物的制备工艺与表征等。采用水溶液搅拌法辅助微波制备纳豆激酶HP-β-CD包合物,采用差热分析、酶活测定等方法对包合物进行鉴定。得到纳豆激酶-HP-β-CD的合格产品。纳豆激酶HP-β-CD包合物制备工艺简单,包合物水溶性强,稳定性高,适合制成注射制剂。
To product high-purity nattokinasc by liquid fermentation, separation and purification. To prepare and identify nattokinase- hydroxypropyl -beta -cyclodextrin inclusion compound and study the preparation and the quality characterization of the inclusion compound. The methods of aqueous solution-stirring with microwave were performed for the manufacturing technique and the characteristics were identified by DSC and enzyme activity determination. The qualified products of nattokinas- hydroxypropyl -beta -cyclodextrin inclusion compound were obtaincd. The preparation technique was convenient, and the product was more water-soluble and stable in the conditions of the high temperature and suitable for injection.
出处
《广东化工》
CAS
2011年第1期10-10,44,共2页
Guangdong Chemical Industry
基金
武汉国家生物产业基地2011年生物产业发展资金项目
关键词
纳豆激酶
羟丙基-Β-环糊精
包合物
nattokinase
hydroxypropyl-beta-cyclodextrin
inclusioncompouud