大剂量百草枯中毒对大鼠消化道损伤的病理观察

Pathological observation of gastrointestinal tract injuries in rats exposed to high dose of paraquat

目的了解大剂量百草枯对SD大鼠消化道损伤的影响,为提高临床治疗效果提供理论依据。方法 20只SD大鼠雌雄各半,随机分为对照组及百草枯组,每组10只。对照组用生理盐水灌胃,实验组用百草枯溶液灌胃,剂量为125 mg/kg,观察大鼠中毒后的行为学变化及30 d内病死率。死亡大鼠取胃、肝、小肠、大肠等器官作病理分析。结果中毒大鼠饮食、饮水少,30 d内病死率为70%。中毒死亡大鼠胃黏膜出现轻度糜烂,肝静脉轻度充血,小肠及大肠黏膜坏死,局部脱落,伴炎细胞浸润。结论大剂量百草枯中毒大鼠消化道出现明显的病理损伤,以小肠及大肠最严重,临床治疗应加强对胃肠道的保护。

Objective To observe the pathological injuries of gastrointestinal tract in rats exposed to high dose of paraquat,and to provide a rationale for the improvement of clinical therapeutic effects.Methods 20 SD rats were randomly divided into control group and paraquat group（n=10）.Control group was intragastrically administrated with 0.9% normal saline,and paraquat group were intragastrically administrated with paraquat at 125 mg/kg.Then behavioral changes and case fatality rate of both groups in 30 d after paraquat poisoning were observed.The stomach,liver,small intestine and large intestine were dissected from dead rats and examined with routine pathological analysis.Results Rats ate and drank became less after paraquat poisoning,and the fatality rate in 30 d was 70%.Dead animals showed mild mucosal erosions in stomach,mild congestion in hepatic venous,mucosal necrosis and partial mucosa shedding in small and large intestine accompanied by inflammatory cell infiltration.Conclusion Serious pathological injuries can be seen in gastrointestinal tract,especially in small and large intestine of rats exposed to high dose of paraquat.Therefore,the protection of the gastrointestinal tract should be strengthened in clinical treatment of paraquat poisoning.