胶束纸色谱法分离和鉴定黄酮类化合物

SeparationandidentificationofflavonoidswithMicellarpaperchromatography

目的：探讨胶束纸色谱在分离、鉴定黄酮类化合物中的应用。方法：采用高于临界胶束浓度的ＳＤＳ、ＢｚＢｒ水溶液作展开剂通过纸色谱法分离和检测桑色素、杨梅黄素等８种黄酮甙和甙元，并对胶束浓度和有机添加剂影响分离效果作了观察。结果：０．０１～０．０２ｍｏｌ／Ｌ的ＳＤＳ或ＢｚＢｒ胶束展开体系适宜于分离黄酮甙类，而当展开剂中０．０６ｍｏｌ／Ｌ的ＳＤＳ水溶液与乙酰乙酸乙酯的体积比为１０∶１时，黄酮甙元可以得到满意的分离效果。结论：胶束纸色谱能简便、快速、准确分离和鉴别黄酮类化合物。

Objiective: ToinvestigatetheuseofMicellarpaperchromatography(MPC)ontheseparationandidentificationofflavonoids. Methods: Morin,Myricetin,Quercetin,Chrysin,Genistein,Hesperidin,NaringinandRutinwereseparatedandidentifiedbySDSorBzBrsolutionabovecriticalmicelleconcentrationonpaperchromatography.Theeffectsofthemicelleconcentrationandratiooforganicadditivewereinvestigated. Results: Toseparateflavoniedglycosides,thebettermicelleconcentrationofSDSorBzBris0.010.02mol/L;thespoteffectsofflavonoidaglyconesonMPCcanbeimprovedwhentheratioof0.06mol/LSDSsolutionandacetoaceticesteris10:1(v/v)inmobilephase. Conclusion: MPCisasimple,rapidandaccuratemothodtoanalyzeandseparateflavonoids.