骨髓增生异常综合征贫血患者Hepcidin的检测意义

Detection of hepcidin in myelodysplastic syndrome patients with anemia and its clinical significance

目的:探讨骨髓增生异常综合征(MDS)贫血患者Hepcidin的表达及其与血清铁蛋白(SF)间的关系,为临床诊治铁紊乱相关的贫血寻找理论依据。方法:采用酶联免疫吸附试验(ELISA)、放射免疫法分别测定45例MDS贫血患者及60例正常对照组血清中的Hepcidin,血清铁蛋白水平。结果:正常对照组中Hepcidin水平与年龄无关,男性高于女性(P<0.01),Hepcidin与SF水平存在较强的正相关性(r=0.895,P<0.01)。MDS组患者与对照组相比,其Hepcidin、SF显著增高(P<0.01);MDS组内(60岁患者Hepcidin、SF显著高于≤60岁患者(P<0.01);输血>3次患者Hepcidin、SF显著高于输血≤3次患者(P<0.01);Hepcidin与SF水平存在较强的正相关性(r=0.983,P<0.01)。结论:生理情况下,Hepcidin主要受SF调节,男性Hepcidin显著高于女性。MDS患者Hepcidin较正常人显著增高,且与年龄和输血次数显著相关。

Objective： To study the relationship of Hepcidin and serum ferritin（SF） in myelodysplastic syndrome（MDS） patients with anemia and normal control group,in order to provide the theoretical basis for clinical diagnosis and treatment of iron disorders related anemia.Methods： EnzymE-linked immunosorbent assay（ELISA） and radioimmunoassay were used to measure serum Hepcidin of 45 cases of MDS patients with anemia and 60 cases of normal control group,serum ferritin levels were also detected.Results： Hepcidin levels in the normal control group had nothing to do with age.Hepcidin of male group was significantly higher than that of female group（P0.01）,and there was a strong positive correlation between Hepcidin and SF level（r=0.895,P0.01）.The Hepcidin level of MDS patients was significantly higher than that of control group（P0.01）;for MDS group,the level of Hepcidin and SF in more than 60-year-old patient group was significantly higher than its level in less than 60-year-old patient group（including 60 years old patients,P0.01）;the level of Hepcidin and SF in the group of patients receiving blood transfusion more than three times was significantly higher than that in the patients with blood transfusion less than three times（including three times,P0.01）.Strong positive correlation was found between Hepcidin and SF levels（r=0.983,P0.01）.Conclusion： In physiological circumstances,Hepcidin was mainly regulated by SF,Hepcidin of male was significantly higher than that of females.Hepcidin in patients with MDS was significantly higher than in normal people,and significantly correlated with the number of blood transfusion and age.There was a strong positive correlation between Hepcidin and SF levels in MDS group.