抗生素和血清制剂治疗小鼠脓毒症时对血浆内毒素和细胞因子的影响

Effect of antibiotics and anti TNF agent on the serum endotoxin and cytokines in the treatment of sepsis in mice

目的通过观察脓毒症小鼠的治疗过程，研究抗生素和血清制剂对血中内毒素脂多糖（ＬＰＳ）和细胞因子的影响。方法脓毒症模型采用ＮＨ１小鼠，腹腔大肠杆菌。治疗分３组，先锋霉素５（ＣＥＦ），新生小牛血清（ＮＢＳ），生理盐水（ＮＳ）。结果ＮＢＳ组的７２小时生存率明显高于ＮＳ组（Ｐ＜０．０５）。ＣＥＦ组３小时白细胞计数低于ＮＳ组（Ｐ＜０．０５）。ＮＢＳ组的１、３小时ＬＰＳ水平低于ＮＳ对照组（Ｐ＜０．０５），血ＩＬ１和ＴＮＦ低于ＮＳ组（Ｐ＜０．０５）。结论抗生素对抑制白细胞升高起主导作用，对ＬＰＳ和细胞因子活性无明显抑制作用。血清制剂可能同时具有抗休克和抗炎症的双重作用，对提高早期生存率起重要作用。

Objective\ To investigate the efficacy of an anti TNF monoclonal antibody (Z12) administrated at different time points in the treatment of septic mice.\ Methods\ NH1 male mice were challenged twice at a 12 h interval with intraperitoneal (IP) bolus injection of E. Coli O111 B4 suspension. Z12 at 0.1 mg per mouse was IP injected at first challenge for early group and at second challenge for later group respectively, and the same volume (0.5 ml) of normal saline (NS) was IP infected as control group. Survival, white blood cells of peripheral blood and cytokines' activities were investigated. Results\ Survival of later group was higher than that of early group ( P <0.01). Both early and later Z12 groups showed lower WBC count and blood cytokine activities than the NS control group ( P <0.05). The TNF and IL 6 levels in the early group were lower than in the later group ( P <0.05). But IL 1 in the early group was higher than in the later group ( P <0.05). Conclusion\ Anti TNF antibody should be applied in later time rather than in early time after infection.