摘要
目的观察腹主动脉阻断所引起的肝、肾、小肠等内脏缺血再灌注损伤的改变。方法建立小猪腹主动脉阻断1小时的模型,检测在不同再灌注时点组织及血液中丙二醛(MDA)和超氧化物歧化酶(SOD)的变化,同时检测肝肾功能和动脉血气分析,观察动物术后的生存情况。结果与缺血前比较,大多数再灌注时点血、组织中MDA明显升高,而SOD明显降低(P<0.05)。在再灌注2小时,血中谷丙转氨酶(ALT)、尿素氮(BUN)、肌酐(CRE)较缺血前明显升高(P<0.01),代谢性酸中毒也极为明显。多数动物术后能够存活,但均出现下肢截瘫。结论腹主动脉阻断1小时能引起明显的内脏缺血再灌注损伤改变,多数内脏经处理后其损伤能够得到代偿恢复,而脊髓损伤恢复困难。
Objective\ To investigate the changes of ischemia reperfusion injury in the liver, kidney and intestine caused by abdominal aorta temporary occlusion. Methods\ A pig visceral ischemia model was established by abdominal aorta cross clamping above the celiac artery for 1h. The changes in superoxide dismutase (SOD) and malondialdehyde (MDA) in the liver, kidney and intestinal tissues and the blood were studied during the different ischemia reperfusion periods. Hepatic and renal functions and blood gas analysis were inspected, and the postoperative survival conditions of the animals were also studied.Results\ MDA levels in the tissues and blood were markedly increased, while the activity of SOD decreased ( P <0.05) during the period of ischemia reperfusion as compared with those before ischemia. At the 2nd h following the reperfusion, the levels of ALT, BUN and CRE in the blood were significantly higher than those of per ischemia ( P <0.01), and the animals also developed a serious metabolic acidosis. Most animals could survive after the operation, but paraplegia occurred in all animals.Conclusion\ Abdominal aorta occlusion had a marked impact on ischemia reperfusion injury of viscera. Injuries of most organs could restore if the period of occlusion was less than 1 h, but the restoration of spinal cord injury was very difficult.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1999年第4期317-318,共2页
Chinese Journal of Experimental Surgery
基金
广东省卫生厅科研基金
关键词
腹主动脉阻断
再灌注损伤
氧自由基
内脏缺血
Abdominal aorta\ \ Ischemia reperfusion injury\ \ Qxygen free radicals\ \ Viscera