RASSF1A和SKP2在胃癌中的表达及与幽门螺杆菌L型感染关系的研究

The expression of RASSF1A and Skp2 in gastric carcinoma and the relationship with Helicobacter pylori L-form infection

目的研究幽门螺杆菌L型(Helicobacter pyloriL-form,H.pylori-L型)感染,Ras相关区域家族1A基因(RASSF1A)和细胞S期激酶相关蛋白2(Skp2)在胃癌发生、发展中的作用及相互关系。方法应用革兰染色和免疫组织化学SP法检测50例胃癌组织及20例癌旁正常组织中的H.pylori-L型感染情况;同时应用逆转录聚合酶链式反应(RT-PCR)和免疫组织化学SP法检测上述组织中癌基因skp2和抑癌基因RASSF1A的表达。结果 50例胃癌组织标本中免疫组化及革兰染色H.pylori-L型同时阳性的病例有30例,胃癌组织和癌旁正常组织中H.pylo-ri-L型阳性率分别为60.0%和20.0%,2组之间差异有统计学意义(P<0.05)。胃癌组织中Skp2表达阳性率明显高于对照组(P<0.01);而RASSF1A表达阳性率明显低于对照组(P<0.01);H.pylori-L型阳性组的Skp2表达阳性率高于H.pylori-L型阴性组(P<0.05);H.pylori-L型阳性与Skp2的表达阳性呈正相关关系;RASSF1A的表达与H.pylori-L型阳性呈负相关关系。结论 H.pylori-L型感染在胃癌的发生发展过程中起重要的作用,其促进胃癌发生、发展的机制可能涉及上调Skp2的表达和下调RASSF1A的表达。

Objective To investigate the role of Helicobacter pylori L-form（H.pylori-L） infection in the genesis and development of gastric carcinoma and the relationship with RASSF1A（Ras association domain famil 1A） and Skp2（S-phase kinase associated protein 2）.Method H.pylori-L was examined in 50 cases of gastric carcinoma and 20 matched normal tissues by means of Gram stain and immunohistochemical staining with SP method.The expressions of RASSF1A and Skp2 were detected in 50 cases of gastric carcinoma by RT-PCR（Reverse transcription-Polymerase chain reaction） and immunohistochemical staining with SP method.Result In these cases with gasric carcinoma,30 cases appeared to be positive for H.pylori-L as revealed both in Gram’s stain and the immunohistochemical staining with SP method;while in the normal controls,the positive rate for H.pylori-L was only 20%（P〈0.05）.The positive rates of mRNA and protein of Skp2 were higher than those in the control group,and RASSF1A expression was significantly lower than that of the control group（P〈0.01）.There was a definite correlation between infection of H.pylori-L and the expression of RASSF1A and Skp2.Conclusion H.pylori-L may play an important role in the process of the occurrence and development of gastric carcinoma.Its mechanism may be related to the up-regulated expressions of Skp2 and the down-regulated expressions of RASSF1A.