糖尿病与非糖尿病牙周炎优势菌的AFM比较研究

Comparison of diabetes-associated periodontitis and nondiabetes-associated periodontitis by AFM

目的运用原子力显微镜(Atomic Force Microscope,AFM)观察牙周炎患者龈下优势菌表面超微形态特征,分析糖尿病牙周炎、非糖尿病牙周炎研究对象菌斑中优势菌表面形态是否存在差异,探讨有致病作用的优势菌形态与两种类型牙周炎病理损伤之间的相关性。方法选取糖尿病牙周炎、非糖尿病牙周炎典型病例各30例,应用AFM对符合设定标准的优势菌进行扫描。测量每个菌体表面平均粗糙度、峰值、隆起平均大小,观察其纳米结构变化,评价两种类型牙周炎龈下优势菌的形态学特征。结果通过研究发现糖尿病牙周炎组、非糖尿病牙周炎组龈下优势菌表面差异存在统计学意义,糖尿病牙周炎组优势菌表面的平均粗糙度、峰值、隆起平均大小均明显高于非糖尿病牙周炎组(P<0.05)。结论糖尿病牙周炎龈下优势菌表面的纳米级超微形态不同于非糖尿病牙周炎,其差异性显示两种类型牙周炎的牙周损伤特点与其各自龈下优势菌的形态结构相关。

Objective To observe ultramicro surface morphological characteristics of subgingival dominant bacteria from periodontitis patients by using Atomic Force Microscope（AFM）,investigte the morphological differences of subgingival dominant bacteria between periodontitis patients with and without diabetes,and explore the correlation between pathological periodontal lesions and pathopoiesis of dominant bacteria from those two groups of periodontitis. Method 30 cases each of diabetes-associated periodontitis and nondiabetes-associated periodontitis were chosen.Dominant bacteria from those cases were scanned by AFM using tapping mode in the range of 1 μm,the surface structure of dominant bacterias being measured in terms of mean roughness,peak value,as well as the average size of eminentia.The morphologic features of the two groups of dominant bacteria were evaluated.Result There were significant differences between the surface of dominant bacteria from the two groups in terms of mean roughness,peak value,and the average size of eminentia.The parameters of diabetes-associated periodontitis were higher than those of nondiabetes-associated periodontitis（P〈0.05）.Conclusion The ultramicro surface morphological characteristics of subgingival dominant bacteria from diabetes-associated periodontitis patients are obviously different from those of nondiabetes-associated periodontitis,which suggests that the characteristics of periodontal lesions is correlated with morphological structure of subgingival dominant bacteria from periodontitis patients.