p16、CyclinD1、Cdk4、Rb基因在胰腺癌中的表达及其意义

The expressions of p16, cyclin D1,Cdk4,Rb, andtheir significance in pancreatic carcinoma

目的：探讨ｐ１６ 、 Ｃｙｃｌｉｎ Ｄ１、 Ｃｄｋ４ 、 Ｒｂ 在胰腺癌中的作用及其相互关系。方法：免疫组织化学检测ｐ１６ 、 Ｃｙｃｌｉｎ Ｄ１、 Ｃｄｋ４ 、 Ｒｂ 蛋白在胰腺癌中的表达，原位杂交检测ｐ１６ 和 Ｃｙｃｌｉｎ Ｄ１ 在胰腺癌中的存在状况。结果：ｐ１６ 在胰腺癌中低表达， Ｃｙｃｌｉｎ Ｄ１ 和 Ｃｄｋ４ 过度表达，未发现 Ｒｂ 在胰腺癌标本中缺失；ｐ１６ 与 Ｃｙｃｌｉｎ Ｄ１，ｐ１６ 与 Ｒｂ 呈负相关关系（ Ｐ＜ ０．０５） ， Ｃｄｋ４ 与 Ｃｙｃｌｉｎ Ｄ１ 呈正相关关系（ Ｐ＜ ０ ．０５） 。结论：胰腺癌发生机制涉及ｐ１６ 、 Ｃｙｃｌｉｎ Ｄ１、 Ｃｄｋ４ 和 Ｒｂ 调节环路中多个基因的异常，ｐ１６ 低表达和 Ｃｙｃｌｉｎ Ｄ１ 过度表达可能起在胰腺癌发生中起协同作用。

Objectives:To study the mechanism of p16, CyclinD1, Cdk4, Rb, and their relationship in pancreatic carcinoma. Methods:To examine these proteins and genes by immunchistochemistry and in situ hybridization. Results:Overexpression of cyclin D1 and Cdk4 were revealed in these samples and p16 was undetectable, the Rb deletion was not revealed in these samples. There is a negative relationship between p16 and cyclin D1,p16 and Rb in these samples (P< 0.05); there is a positive relationship between cyclin D1 and Cdk4 (P< 0.05). Conclusions:Abnormality of many genes involved in the regulatory pathway of p16,cyclin D1/Cdk4 and Rb may be involved in the molecular mechanism of pancreatic carcinogenesis, both the lower pression of p16 and the overexpression of cyclin D1 may coexist in the development of pancreatic carcinoma.