一个玉米Mlo基因的电子克隆与生物信息学分析

Analysis of Electronic Clone and Bioinformatics on Mlo Gene from Maize

Mlo基因家族在植物抗病方面有极大的优势,但有些Mlo基因的功能还未知。经序列拼接电子克隆得到1个玉米的Mlo基因,采用生物信息学方法预测分析了编码蛋白的一、二、三级结构,并对其功能进行了预测。结果表明:玉米Mlo基因编码的蛋白有一个保守的DUF1084结构域,此结构域功能在植物中尚未知。生物信息学分析表明,此蛋白很可能是一种类似于G蛋白偶联受体的膜结合转运蛋白而参与到信号传递过程中。

Mlo gene family has great advantages in plant disease resistance, but some of the Mlo gene function is unknown yet. A Mlo gene was obtained by sequence splicing and electronic cloned from Zea mays（Maize）. The primary, secondary and tertiary structure also the function of the encoded protein were analyzed and predicted by bioinformatics analysis. The results showed that the encoded protein had a conserved domain named DUF1084 but its function was unknown yet in plant. Bioinformatics analysis showed that the encoded protein may be a membrane-bound transporter protein similar to the G protein-coupled receptor and participate in the process of signal transduction in plant.