高密度培养重组E-coli产胆固醇氧化酶的乳糖诱导策略

High cell density culture of recombinant E.coli producing cholesterol oxidase as lactose induced strategy

研究了乳糖诱导重组大肠杆菌表达胆固醇氧化酶。结果表明,乳糖不仅可以作为诱导剂诱导外源蛋白的合成,而且能作为碳源促进菌体的生长。通过对诱导条件的优化,实现了重组大肠杆菌的高密度培养,最高密度达68.9(OD600);在此基础上确定了最佳的诱导时机为发酵中期,菌体产酶水平达6005.66U/L,生产强度为200.19U/L.h,实现了胆固醇氧化酶的高效生产。

The high cell density culture of recombinant E.coli producing cholesterol oxidase as lactose induced strategy was studied. It was found that lactose could induce foreign protein expression and enhance cell growth during the induction period. Through proper optimization of culture and induction conditions,the cell density reached 68.9（OD600）. At the basic,the best induction time was identified as intermediate cell density. Under the optimized cultivation condition,the highest cholesterol oxidase activity（6005.66U/L）and highest cholesterol oxidase productivity（200.19U/L·h）were achieved.