酶法制取橙皮果胶的研究

Cellulase extraction of pectin from orange peel

纤维素酶酶系组成复杂,不同来源纤维素酶在果胶提取中表现出的作用不同。采取与前人研究不同的纤维素酶对橙皮果胶进行提取,对三种不同来源的纤维素酶比较分析,筛选出一种价格低廉、适合果胶提取的纤维素酶,并采用四元二次通用旋转组合设计方法研究了其提取果胶工艺中缓冲溶液的pH、提取时间、酶添加量、提取温度对果胶得率的影响;建立数学模型,寻求测定条件的最优组合;以DesignExpert软件进行分析,模型经检验差异显著,失拟检验不显著,具有良好的统计学意义,最优组合为:酶添加量3.38mL、时间6.66h、温度47.5℃、pH4.95,预测模型果胶得率为14.43%,验证实验得率为14.79%,大大提高了纤维素酶制取果胶的得率,远远高于文献报道,并且酶的价格低廉,大大降低了生产成本。

The characteristics of cellulase system is complicated,cellulase from different sources act with different effects in pectin extraction. The extraction of pectin from orange peel by cellulases which was different from the ones in literatures was studied. One kind of low-cost cellulase suitable for pectin extraction was screened out from three kinds of cellulases. And the effects of extraction pH,temperature,time,enzyme concentration on the pectin yield were studied by quadratic regression rotational composite design. The best effective factors were determined. Via statistical software of Design Expert analysising,the model was significant and the lack of fit was not significant with obvious statistical significance. The optimal combination forecasting model was drew on volume of enzyme 3.38mL,time 6.66h,temperature 47.5℃,pH4.95,pectin yield of forecasting model was 14.43% and verification experiment was 14.79%. Pectin yield was improved greatly and much higher than the reported in literatures,and cellulose was low-cost,so cost of production can be saved greatly.