摘要
以大豆磷脂为原料,LipozymeTLIM为酶试剂,正己烷为溶剂,棕榈酸为酰基供体,对大豆磷脂和棕榈酸进行酶法酸解研究。通过实验得出较优的反应条件:液料比(正己烷/磷脂)5∶1,底物摩尔比(棕榈酸/磷脂)5∶1,加酶量(以底物总量为准)20%,反应温度65℃,反应时间60h,水分添加量(以酶量为准)2%,该条件下验证得磷脂中棕榈酸的含量可达到34.45%。
With soybean phospholipids as raw materials,palmitic acid as acyl donor,Lipozyme TL IM as enzyme reagent and hexane as solvent,soybean phospholipids with palmitic acid acidolysis was prepared using lipase-catalyzed. The optimal reaction conditions were obtained through experiment：phospholipids concentration（hexane/phospholipids）5∶1,substrate ratio（palmitic acid/phospholipids）5∶1,lipase dosage（based on the total substrates）20%,reaction temperature 65℃,reaction time 60h,and water addition（based on lipase）2%. Incorporation of palmitic acid was up to 34.45%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2011年第2期236-239,244,共5页
Science and Technology of Food Industry
基金
江苏省科技型中小企业创新资金(BC2008021)
关键词
大豆磷脂
棕榈酸
脂肪酶
酸解
soybean phospholipids
palmitic acid
lipase
acidolysis
