青藏高原藏木香总黄酮提取工艺研究及含量测定

Study on the extraction technique and quantification of total flavonoids in Inula racemosa grown in Qinghai-Tibet plateau

目的:研究藏木香中总黄酮的提取工艺及含量测定。方法:采用正交实验进行优选,考察乙醇浓度、溶剂倍数、提取时间等因素对黄酮得率的影响,用紫外分光光度法测定其含量。结果:乙醇浓度是主要的影响因素,最佳提取工艺为选用75%乙醇作为提取溶剂,料液比1∶20,水浴热回流提取2h。以芦丁为对照品,采用分光光度法测定出总黄酮平均含量为0.28mg/g。结论:提取工艺可行;紫外分光光度法简单、快速、准确;为藏木香深度开发提供了科学依据。

Objective：Aim to study the best conditions of extraction techniques for total flavonoids in Inula racemosa. Methods：The best conditions of extraction technique for total flavonoids were optimized with orthogonal experiment. Developed by ethanol solution with different concentrations,solvent multiples and extraction duration,determination of total flavonoids with UV-spectrophotometry was made. Results：Ethanol concentration was the major factor for determining total flavonoids contents. The best conditions for the extraction techniques of total flavonoids were：75% ethanol which was 20 times of the medicine amount,reflux extracting 2 hours. The yield of total flavonoids in Inula racemosa was estimated at 0.28mg/g. Conclusion：The extraction techniques are feasible and the UV-spectrophotometric method is simple,quick and accurate.