桑葚多糖提取、纯化分离及其降糖作用的研究

Study on the purification and hypoglycemic effect of polysaccharide in mulberries

目的:探讨桑葚多糖纯化工艺及降糖作用。方法:AB-8树脂纯化,乙醇醇沉,5%的三氯醋酸的正丁醇+氯仿正丁醇(5∶1)去蛋白,所得多糖经DEAE-纤维素柱分离,所得多糖进行降糖效果测定。结果:实验表明,树脂纯化、乙醇醇沉,5%的三氯醋酸的正丁醇+氯仿正丁醇(5∶1)去蛋白纯化效果较好,DEAE-纤维素柱分离中性多糖P-1,酸性多糖P-2、P-3,且降糖效果明显。结论:该纯化工艺和药理实验为桑葚的综合利用提供了新途径。

Objective：To explore the purification and hypoglycemic effect of polysaccharide in mulberries. Methods：AB-8 resin purification,ethanol precipitation,5% trichloroacetic acid of the n-butanol＋chloroform n-butanol（5∶1）to remove protein,polysaccharide obtained was isolated by DEAE-cellulose column separation for hypoglycemic effect test. Results：The tests showed that resin purification,ethanol precipitation,5% trichloroacetic acid of the n-butanol＋chloroform n-butanol（5∶1）removed protein absolutely,neutral polysaccharides P-1,acidic polysaccharide P-2,P-3 can be isolated by DEAE-cellulose column separation effectively,the polysaccharide also had remarkable hypoglycemic effect. Conclusion：The technology method provided a new approach for the comprehensive utilization of mulberries.