莲子红皮多糖提取工艺研究

Study on extraction technology of polysaccharides from red skin of locus seed

红皮莲子在磨皮加工中会产生约15%的红皮粉。经测定,红皮粉中总多糖的含量为9.90%。为进一步开发和利用莲子的营养价值,本研究采用水提醇析法制备莲子红皮多糖。在单因素实验分析基础上,以多糖得率为指标,设计正交实验对提取条件进行了优化。结果表明,使用α-淀粉酶从莲子红皮中提取多糖,当料液比为1∶15,酶解时间为105min,粉碎粒度为100目,多糖得率可达8.42%,多糖纯度为83.19%。由此可见,莲子红皮粉具有可开发利用的良好前景。

Red skin seeds of lotus usually produce about 15% of the red skin powder in the resurfacing process. It was determined that the polysaccharide content in red skin of lotus seed was 9.90%. For further developing the use value of lotus,the present study was designed to extract polysaccharides from the red skin powder using water-extraction and ethanol-precipitation method. With the polysaccharide yield as the index,the extraction conditions were optimized by the orthogonal-array-design on the basis of single-factor experiments. The results showed that the optimal conditions for extracting polysaccharides with α-amylase were as following：the particle size 100 mesh,the material/liquid ratio 1∶15,and the enzymolysis time 105min. Under the above conditions,the polysaccharide yield was 8.42%,and the purity of polysaccharide was 83.19%. It indicated that the red skin of lotus seeds had good development prospects.