皋茶急性毒性安全性评价研究

Acute toxicity evaluation of Gaocha

目的:通过1次大剂量给药,观察小鼠出现的毒性反应及死亡情况,考察皋茶应用的安全性。方法:依据GB15193-2003《食品安全性毒理学评价程序和方法》进行急性毒性实验,皋茶水提物小鼠灌胃给药0.4mL/10g体重,灌胃给药1次,药后正常饲养,连续观察14d。结果:皋茶水提物小鼠灌胃最大给药量为22.8g浸膏/kg,相当于临床60kg成人拟定日用量275倍。结论:皋茶水提物口服毒性低,应用较安全。

Objective：Through once large dose feeding Gaocha and observing the rapid toxicity effect on the mice,the safety of Gaocha was studied. Methods：The test of acute toxicity according to GB15193-2003（Appraisal process and Methods of Toxicity of Food Safety）was carried out to research the toxicology of food of the Gaocha. The mice were feed Gaocha with 0.4mL/10g each time,after that were feed normal. Then observed and recorded in the next 14d. Results：There was no evident poisoning symptom or any death for animals after test on the young mice of different sex which had eaten the Gaocha with 22.8g/kg. Be equivalent to that 275 times of adult drink amounts a day. Conclusion：It is concluded that the Gaocha is highly safe for drinking.