摘要
在分析高效液相色谱法(HPLC)和紫外分光光度法(UV)的基础上,通过运用Origin8.0数据处理分析软件,建立了一种快速、有效、准确的葵粕绿原酸(CGA)检测方法。结果表明:这两种方法测定的已知浓度CGA的含量符合方程:Y=1.386+0.4082X+0.0132X2-8.438X3,R2=0.9856,其中X为UV测定结果,Y为HPLC测定结果,并通过验证,在5.5～33μg/mL浓度范围内,该方程准确可靠,从而可以实现UV法快速、准确地测量大批量样品中的CGA含量。
In order to create a kind of quickly,effective and precise chlorogenic acid（CGA）determination method,on the basis of HPLC and UV methods,Origin8.0-statistical analysis software was adopted to build a fitted equation with HPLC results as Y value,and with UV result as X value. The equation was obtained as：Y=1.386＋0.4082X＋0.0132X2-8.438X3,R2=0.9856. According to confirmatory test results,in the 5.5~33μg/mL concentration range,the fitted equation was precise and reliable. And so the modified UV method can determine CGA quickly,precisely and with large quantities.
出处
《食品工业科技》
CAS
CSCD
北大核心
2011年第2期341-343,346,共4页
Science and Technology of Food Industry
基金
中国农业科学院作物科学研究所中央级公益性科研院所基本科研业务费专项资助项目
关键词
绿原酸
UV法
HPLC法
检测
chlorogenic acid
UV method
HPLC method
determination
