摘要
应用冷冻结晶技术,对共轭亚油酸两种主要异构体c9,t11 CLA和t10,c12 CLA进行分离。结果表明,通过尿素包合法,去除原料中的饱和脂肪酸,然后在乙醇作溶剂情况下,将乙醇与共轭亚油酸乙酯按18:1配比,置于-56℃低温冷柜中,反应96 h后得沉淀结晶产品,其所含的t10,c12 CLA比c9,t11 CLA高达12.28:1,共轭亚油酸总含量为95.17%。
The conjugated linoleic acid two main isomers were separated by freezing crystallization.At first the saturated fatty acid was removed by urea adduction fractionation,then the conjugated linoleic acid was frozen at-56 ℃ in ethanol,to improve the ratio of two isomers.At the optimal conditions: ethanol dosage 18∶1(based on CLA),after reaction 96 h,t10,c12CLA: c9,t11 CLA=12.28∶1,and the production of CLA contented in ethyl ester was up to 95.17%.
出处
《食品与机械》
CSCD
北大核心
2011年第1期26-28,共3页
Food and Machinery
基金
国家高技术研究发展计划"863"资助课题(编号:2007AA091905)
关键词
共轭亚油酸
异构体
冷冻结晶
congjugated linoleic acid
isomers
freezing crystallization