基于L-Cys/MWCNT/HRP修饰金电极的过氧化氢传感器

Hydrogen Peroxide Biosensor Based on L-cysteine/Multi-walled Carbon Nanotube/Horseradish Peroxidase Modified Glod Electrode

制备了以L-半胱氨酸(L-Cys)、多壁碳纳米管(MWCNT)、辣根过氧化物酶(HRP)修饰的H2O2生物传感器。研究了修饰电极的电化学特性,探讨了测定溶液PBS的温度和pH值对电极响应H2O2的影响,考察了电极的重现性、抗干扰能力及使用寿命等。结果表明:该传感器具有线性范围宽、检出限低、灵敏度高、稳定性好和抗干扰能力强等特点。

Horseradish peroxidase（HRP） was selected as a model enzyme to fabricate a novel biosensor based on the L-cysteine,mutli-walled carbon nanotube.The electrochemical characteristics of the electrode were characterized by cyclic voltammetry.And the effects of pH and temperature of phosphate buffer solution on the response to H2O2 were also studied.At last,the reproducibility and selectivity of the modified electrode were investigated in detail.The results show that the modified electrode exhibites wide linear range,lower detection limit and good stability,as well as certain anti-interference ability.