黄连的特异性、一致性和稳定性测试性状的选择

The selection and analysis of characteristics of Coptis in distinctness,uniformity and stability test

目的对四川主产黄连进行了测试性状的选择与初步分析,为制订黄连特异性、一致性和稳定性(DUS)测试指南提供科学依据。方法选择黄连的48个形态性状,包括12个必测性状和36个补充性状,分析数量性状的差异性;探讨HPLC指纹图谱在黄连DUS测试应用,分析了不同品种的黄连差异性,作为鉴定辅助手段。结果黄连的数量性状受环境与生长年限影响大,许多性状均有较大变异。采用HPLC-FPS曲线图分析不同品种的黄连,差异性都比较明显。结论制订测试指南时应以植物形态特征为主,品质性状和生理生化性状为辅。如果形态性状难以区分,可将化学成分指纹图谱技术作为辅助鉴定手段。

OBJECTIVE To select and analyze the characteristics of Coptis chinensis Franch.grown in Sichuan province,and supply certain theoretical basis of Guidelines for the conduct of tests for distinctness,uniformity and stability of Coptis.METHODS 48 morphological characteristics including 12 necessary characteristics and 36 additional characteristics were selected,and the varieties of quantitative characteristics were analyzed.HPLC finger printing was employed to analyze the variance of different Coptis varieties.RESULTS There were large variations in several characteristics the number of scape,the number of rhizome branch.The performance of consistency and stability were different,which was affected by environment and growth years.Significant differences were observed among different coptis varieties by using HPLC-FPS.CONCLUSION To develop testing guidelines,morphological characteristics should be considered firstly,quality characteristics,physiological and biochemical characteristics secondarily.HPLC finger printing should be employed as a method for supporting identification if it is difficult to distinguish morphological characteristics.