HPLC测定大鼠血浆中的三七皂苷R_1和人参皂苷Rg_1、Rb_1

Determination of notoginsenoside R_1 and ginsenoside Rg_1,Rb_1 in rat plasma by HPLC

目的采用HPLC测定大鼠血浆中三七皂苷R1和人参皂苷Rg1、Rb1的含量。方法以淫羊藿为内标,采用C18色谱柱,乙腈-水线性梯度洗脱,流速1.0 mL.min-1,柱温30℃,检测波长203 nm。结果样品中的3种皂苷成分分离良好,且线性关系良好;R1、Rg1和Rb1的线性范围分别为2～200、5.5～550、10.5～1050μg.mL-1;检测限分别为0.6、0.55、0.79μg.mL-1。结论所建方法操作简单、结果准确可靠,可用于测定大鼠体内三七皂苷R1和人参皂苷Rg1、Rb1的含量。

OBJECTIVE To establish an assay method for the determination of notoginsenoside R1 and ginsenoside Rg1、Rb1 in rat plasma.METHODS Icariin was used as the internal standard.C18 column was used.The mobile phase was acetonitrile-water gradient elution,flow rate was 1.0 mL·min-1,the column temperature was 30 ℃ and the detected wavelength was at 203 nm.RESULTS Three saponins were separated well.A good linear relationship was obtained with the range of 2-200 μg·mL-1 for notoginsenoside R1,5.5-550 μg·mL-1 for ginsenoside Rg1 and 10.5-1050 μg·mL-1 for ginsenoside Rb1.The limit detection was 0.6,0.55,0.79 μg·mL-1,respectively.CONCLUSION The method is simple and accurate for analysis of notoginsenoside R1 and ginsenoside Rg1、Rb1 in rat plasma.