HPLC同时测定穿心莲药材及其制剂中的6种内酯类成分

Simultaneous determination of six lactones in Andrographis paniculata materials and related preparations

目的采用HPLC法同时测定穿心莲药材及其制剂中6种内酯类成分的含量。方法采用Kromasil RP-C18柱(250mm×4.6 mm,5μm),流动相为乙腈-水,采用二元梯度洗脱,流速1.0 mL.min-1,检测波长226 nm,柱温25℃。结果穿心莲内酯、异穿心莲内酯、新穿心莲内酯、去氧穿心莲内酯、脱水穿心莲内酯和穿心莲宁的平均加样回收率分别为100.5%、100.7%、99.3%、100.8%、100.9%、101.3%;线性范围分别为1.68～67.20μg(r=0.9970)、0.40～16.60μg(r=0.9967)、1.08～43.20μg(r=0.9964)、1.08～43.20μg(r=0.9969)、1.90～76.00μg(r=0.9965)、0.31～12.40μg(r=0.9972)(n=6)。结论所建方法简便可行、重复性良好,可用于穿心莲药材及含穿心莲制剂的质量监控。

OBJECTIVE To develop an RP-HPLC method for simultaneous determination of andrographolide,dehydroandrographolide,deoxyandrographolide,neoandrographolide,ninandrographolide,homoandrographolide in Andrographis paniculata Nees materials and related preparations.METHODS The samples were separated on a Kromasil RP-C18 colum（250 mm ×4.6 mm,5 μm）with the gradient elution of acetonitrile-water at a flow rate of 1.0 mL·min-1.The wavelength was at 226 nm.The column temperature was 25 ℃.RESULTS The standard calibration curves of andrographolide,homoandrographolide,neoandrographolide,deoxyandrographolide,dehydroandrographolide and ninandrographolide were linear in the range of 1.68-67.20 μg（r=0.9970）,0.40-16.60 μg（r=0.9967）,1.08-43.20 μg（r=0.9964）,1.08-43.20 μg（r=0.9969）,1.90-76.00 μg（r=0.9965）,0.31-12.40 μg（r=0.9972）,respectively.The average recoveries of the method were 100.5%,100.7%,99.3%,100.8%,100.9%,101.3%,respectively.CONCLUSION The method was successfully applied to determine the six lactones in Andrographis paniculata collected in different places.It is helpful to control the quality and technonical improvement of Andrographis paniculata and its related preparations.