日本血吸虫(中国大陆株)谷胱甘肽转移酶基因扩增及克隆

Amplification and cloning of Glutathione-S-Transferase gene of Schistosoma japinicum (Chinese Stain)

以日本血吸虫成虫ＲＮＡ为模板逆转录合成ｃＤＮＡ链，设计合成引物，用ＰＣＲ法扩增谷胱甘肽转移酶（ＧＳＴ） 基因编码序列，将其克隆入ｐＧＥＭ—Ｔ载体，并进行初步鉴定。ｐＧＥＭ—Ｔ—ＧＳＴ克隆载体的成功构建，为进一步选择在不同表达系统中表达ＧＳＴ及其在血吸虫病免疫诊断。

SjcDNA first strand synthesis was driven by superscript Ⅱ RT using adult worm RNA as template.Specific primers was designed and synthesized.Coding region gene of SjGST was amplified by PCR.The product from PCR was cloned into pGEM-T-GST vector.The cloning vector pGEM-T-GST was identified by restriction analysis and PCR.The results demonstrated that pGEM-T-GST was successfully constructed and provided the basis for further study on GST expression and its immunological diagnosis and prevention.