骨伤凝胶膏剂中促渗剂的优选研究

Optimization of penetrating agents in Gushang Gel Paste

目的优选骨伤凝胶膏剂的促渗剂,研究其透皮吸收行为和体外释放度。方法采用改良Franzs扩散池法,生理盐水为接受液,用高效液相色谱法测定,以柚皮苷24 h累积透过量和增渗倍数为指标,优选骨伤凝胶膏剂的促渗剂并测定其释放度和透皮吸收情况。结果选用氮酮和丙二醇为促渗剂时,透皮吸收较好,最后确定氮酮和丙二醇的比例为(1∶1),用量为5%;柚皮苷24 h累积释放率为83.48%,累积透皮吸收率为46.59%,体外释药和透皮吸收均符合威布尔分布方程。结论骨伤凝胶膏剂加入氮酮和丙二醇后,透皮吸收明显增加,透皮吸收和释药均良好。

Objective To optimize the penetrating agents for Gushang Gel Paste,and study their percutaneous absorption behavior and release rate in vitro.Methods Franzs diffusion cell test and HPLC were applied to optimize the penetrating agents for Gushang Gel Paste and their percutaneous absorption and release rate were detected taken normal saline water as reception liquid,and 24-hour accumulative osmosis dose and anatonosis times of naringin as indexes.Results When taking azone and propylene glycol as penetrating agents the percutaneous absorption was good,and finally the ratio of azone to propylene glycol was set at 1∶1 with a dose of 5%.The accumulative release rate of naringin was 83.48% and accumulative percutaneous absorption was 46.59%.The release in vitro and percutaneous absorption all accorded with Weibull distribution equation.Conclusion When azone and propylene glycol are added into Gushang Gel Paste,the percutaneous absorption of which is increased significantly,and the percutaneous absorption and release rate are favorable.