出芽短梗霉色素变异菌株R45的普鲁蓝糖发酵研究

STUDIES ON PRODUCING PULLULAN BY AUREOBASIDIUM PULLULANS

用正交实验确定了出芽短梗霉（Aureobasidiumpullulans）色素变异菌株R45的发酵优化条件。在此条件下，摇瓶培养的普鲁蓝糖产量最高可达82．4g／L，转化率为54．9％。实验表明，CaCO3是多糖发酵的重要影响因素，多糖的合成与发酵pH值及细胞形态密切相关。

Fermentative condition of producing pullulan by Aureobasidium pullulans was studied byorthogonal test. In shaking flask, a culture containing 82. 4g/L of pullulan was obtained, the rateof conversion of sucrose to pullulan was 54. 9 %. An important factor of producing pullulan is CaCO3, in addition, pH and cell forms were involved in pullulan synthesis.