氧化应激损伤反应在冠心病心肌缺血血瘀证中的实验研究

Experimental study on oxidative stress response in coronary artery disease (myocardial ischemia) based on the blood stasis syndrome

目的:观察氧化应激损伤反应在冠心病心肌缺血血瘀证中的变化规律。方法:ELISA测定氧化应激关键分子超氧化物歧化酶(SOD)、丙二醛(MDA)、细胞色素C(Cyto C)、一氧化氮(NO)、肿瘤坏死因子(TNF-α)等浓度。结果:氧化应激损伤反应贯穿了血瘀证的始终,与血瘀证密切相关的炎症因子TNF-α与反应内皮功能损伤程度的NO变化趋势与氧化应激损伤程度一致。结论:氧化应激反应参与了血瘀证早期的形成,并诱导了炎症反应与内皮功能的障碍,而Cyto C能比较敏感地反应氧化应激损伤的程度。

Objective： To observe the dynamic changes of indicators related to the oxidative stress response in coronary heart disease （myocardial ischemia） of blood stasis. Methods： Elisa was applied to determine the concentration of key molecules in oxidative stress such as superoxide dismutase （SOD） , malondialdehyde （MDA） , cytochrome C （Cyto C ） , nitric oxide （ NO ） , tumor necrosis factor （ TNF-α ）. Results ： Oxidative stress injury was permeated with blood stasis syndrome from beginning to end. At the same time, TNF-α as an inflammatory cytokines biomarkers and NO as endothelial function were also tested. Their coneentrations have great consistent with the degree of oxidative stress injury. Conclusion ： Oxidative stress involves in the early formation of blood stasis and induces the inflammatory response and endothelial dysfunction, interaction among them may determine evolution and transformation of the blood stasis syndrome. The oxidative stress response of Cyto C can be more sensitive response to oxidative stress level.