葛根素对血管平滑肌细胞周期相关蛋白表达的影响

Effect of Puerarin on cyclin related proteins of vascular smooth muscle cells

目的:研究葛根素对氧化低密度脂蛋白(oxLDL)诱导增殖的血管平滑肌细胞(vascular smooth musclecell,VSMC)细胞周期的影响及其相关机制。方法:建立oxLDL诱导的兔VSMC增殖模型,与不同浓度的葛根素共同培养48h后,用流式细胞仪检测VSMC细胞周期变化及细胞周期调控相关蛋白E(cyclin E)和p27的表达。结果:葛根素组G0/G1期细胞数明显增多,S期细胞数明显减少,与LDL组相比差异显著(P<0.01);cyclin E表达量随葛根素剂量增加而减少,p27蛋白表达量则明显增加。结论:葛根素抑制VSMC增殖,其机制与下调cyclin E、上调p27蛋白表达有关。

Objective ： To investigate The effect and possible mechanism of Puerarin on the cyclin related proteins of oxLDL induced proliferating vascular smooth music cells. Methods ：To establish rabbits＇ proliferating VSMC model induced by oxLDL, incubate the model with different concentrations of Puerarin for 48 hours, then test the cell cycle, cyclin E and p27 by flowmetry. Results：the amount of GO/G1 stage cells increased in puerarin group while the S stage cells decreased and it has a significant difference between the groups（ P 〈 0.01 ）. The expression of cyclin E decreased while the expression of p27 increased in the puerarin group. Conclusion：puerarin inhibit the proliferation of VSMC, the mechanism may related to cyclin E and p27.