一株芴降解菌的分离鉴定及其对多环芳烃的降解广谱性研究

Research on isolation and identification of a fluorene-degrading strain and its broad-spectrum property for degradation of polycyclic aromatic hydrocarbons

从某焦化厂活性污泥中筛选出一株芴的优势降解菌株W-1,经形态学观察、生理生化实验和16S rDNA基因序列分析,确定W-1为粪产碱杆菌。在接种量10%(V/V),初始芴浓度40 mg/L,pH7.0,温度30℃的条件下,接种培养11 d后,菌株W-1对芴的降解效率达到87.8%。菌株W-1对多环芳烃的降解具有广谱性,在分别含有20 mg/L的屈艹(chrysene)、芘、蒽和菲的培养体系中,该菌对屈艹、芘、蒽和菲的降解效率分别可达95.5%、71%、85.7%和80%。研究结果表明,该菌可作为修复多环芳烃复合污染环境的优良微生物资源。

A predominant fluorene-degrading strain W-1 was isolated and screened from activated sludge of a coking plant.The strain was identified as Alcaligenes faecalis by the results of morphological observation,physio-biochemical test and 16S rDNA gene sequence analysis.Under the conditions of inoculation amount of 10%（V/V）,initial concentration of fluorene of 40 mg/L,pH 7.0 and temperature of 30 ℃,the degradation efficiency of fluorene by the strain W-1 reached 87.8% within 11 days.The degradable PAHs as substrates for metabolism of the strain W-1 are diversiform.In the cultivation system solely containing chrysene,pyrene,anthracene or phenanthrene of 20 mg/L,the degradation efficiency of chrysene,pyrene,anthracene and phenanthrene by the strain W-1 reached 95.5%、71.0%、85.7% and 80.0%,respectively.The results showed that the strain W-1 could be used as superordinary microorganism resource to remedy the environment complexly polluted by polycyclic aromatic hydrocarbons.