福建宁化林区动物感染斑点热群立克次体调查研究

Study on the Animals of SFGR in Ninghua County, Fujian

目的 :调查宁化林区动物感染斑点热群立克次体 (SFGR)情况。方法 :鼠类、野兔、野猪、麂、狐狸、狗、牛血清学检测 ,采用微量室温补体结合法 (mCF) ;病原分离 :用鸡胚卵黄囊感染法分离SFGR ,并用mCF、PCR/RFLP技术对分离株进行鉴定。结果 :鼠类、野兔、野猪、麂、狐狸、狗等动物存在SFGR感染 ,其抗体阳性率分别为 11.2 0 %、5 .88%、5 0 .0 0 %、2 0 .0 0 %、5 5 .5 6%、6.67% ,牛血清中未检出SFGR抗体。查出在上述动物体表寄生的蜱类 6属 13种 ,鉴定分类 5 0 0 0余只 ,先后从野兔、麂体表寄生的越原血蜱中分离出 2株SFGR病原体。结论 :从宿主动物、媒介蜱、病原学上进一步证实福建宁化存在斑点热群立克次体自然疫源地。

Objective: To investigate something about the animals infected by spotted fever group rickkettsiosis(SFGR) in Ninghua County, Fujian. Methods: We detected serum antibody of rattus,hare, wild boar,muntiacus reevesi ogilby, fox, dog and cattle with mCF method. SFGR were isloated from Ixodiodeas with Embryonate hens eggs and identified with mCF and PCR/RFLP. Result:The SFGR infection rates of rattus, hare, wild boar, muntiacus reevesi ogilby, fox and dog were 11.20%, 5.88% 50.00% 20.00%, 55.56% and 6.67%, but the antibody of SFGR was not found in serum of cattle. Thirteen species of 6 group of Ixodiodeas were detected on the surface of above animal bodys and about 5 000 of them were identified. Two strain of SFGR were isolated from the haemaphysalis yeni parasitized on the hare and muntiacus reevesi ogilby. Conclusion: It has been proved that there were some nature foci of infections in Ninghua county by host animal, intermedium Ixodiodeas and pathogeny.