我国烟蚜种群分化的RAPD分析

RAPD-PCR ANALYSIS OF POPULATION DIFFERENTIATION OF GREEN PEACH APHID IN CHINA

用RAPDPCR技术研究了我国烟草上烟蚜Myzuspersicae(Sulzer)的种群分化。结果表明:我国烟蚜的不同地理种群和不同体色之间DNA均呈现出多态性,且不同的引物可在不同水平上反应出烟蚜种群的DNA多态性。Nei的遗传距离表明我国烟草上烟蚜的分化仅在种群水平上,并未达到亚种水平。用相似性指数和Nei的遗传距离对所筛选的三个引物OPX04、OPX06和OPX19的扩增结果进行聚类,虽然二者的结果不一致,但均可反应出烟蚜的地理种群和体色生物型的DNA差异。在DNA水平上,与完全周期生活史的烟蚜相比,完全周期和不完全周期生活史混合发生的烟蚜与不完全周期生活史的烟蚜更为接近,而两种生活史混合发生的烟蚜之间无明显差异。从体色看,红色比黄绿色更接近褐色。

RAPD PCR was used to detect the population differentiation of green peach aphid, Myzus persicae (Sulzer) on tobacco from different regions in China, and the results showed that the use of different primers in the RAPD method permits detection of different levels of DNA polymorphism in the aphid's population. Nei's genetic distance ( D ) shows that the differentiation of the aphid from tobacco is only at population level instead of subspecies or species level. Three primers screened from nineteen were used for cluster analysis based on similarity index ( SI ) and Nei's genetic distance (D ). Although there exist some differences between the cluster results of SI and D, they both can show the DNA differences of geographic populations and color forms of the aphid. At DNA level, compared with the holocyclic aphids(from northern China), the aphids mixed with holocyclic and anholocyclic life cycles (from central China) are more similar to the anholocyclic aphids(from southern China), but there is no obvious difference among the aphids from central China. The results also show that, compared with yellowish green forms, the red forms are more similar to the brown ones of the aphid.