虹鳟生长激素cDNA在酵母中的表达

Expression of Rainbow Trout Growth Hormone cDNA in Yeast Saccharomyces cerevisiae Suppored by Key Project on Fish Science and Technology of Agriculture Ministry (No.95 B 96 02 02 04),and the Fund of CAFS (No.95 08 01).

采用聚合酶链式反应（ Ｐ Ｃ Ｒ） 技术对虹鳟生长激素ｃ Ｄ Ｎ Ａ 进行改造。将改造后的基因克隆到含酵母 Ｐ Ｇ Ｋ 启动子的大肠杆菌酵母穿梭质粒ｐ Ｍ Ａ９１ ，转化酿酒酵母 Ｙ３３ ，构建表达鱼生长激素的酵母工程菌 Ｙ３３（ｐ Ｍ Ａｒ Ｇ Ｈ１６） ，并在酵母中获得表达，表达量约占细胞可溶性蛋白总量的３ ％ 。表达产物作为饲料添加剂投喂罗非鱼。

Rainbow trout growth hormone cDNA was modified by PCR.Then the cDNA was subcloned into the expression vector pMA91,and transformed into yeast Saccharomyces cerevisiae Y33 to construct an expression strain Y33(pMArGH16).The recombinant gene could highly express the growth hormone peptide (about 3% of the total proteins) in Y33 (pMArGH16).The expression product was used as a supplement to feed Tilapia fingerling.The result showed that the recombinant fish GH has notable growth enhancement to Tilapia fingerling.