摘要
p53 gene is a tumor suppressor gene and is involved in cell growth control.Wild type p53 gene was inserted into pCA13 and got recombinant plasmid pCAp53.293 cells(a kind of adenovirus packing cells)was co transfected with pCAp53 and pBHG11,recombinant p53 adenovirus was obtained.Virus particle can be seen under electric microscopy and its diameter is about 80 nm.Virus titer is 6.7×10 8 PFU/mL.Recombinant p53 adenovirus have no influence on growth of normal lung cells,but it can cause death of bladder tumor cell (EJ)and primary culture breast cancer cell.MTT assay results showed that recombinant p53 adenovirus inhibited growth of p53 deficient tumor cell,such as bladder tumor cell(EJ)and epidermis cancer cell(A431)and inhibiting rate in 72 hours may be 86.7% and 78.4%.Flow cytometry test showed that recombinant p53 adenovirus induced apoptosis of tumor cell.Wild type p53 gene mediated by adenovirus vector may be used in the treatment for cancer.
p53 gene is a tumor suppressor gene and is involved in cell growth control.Wild type p53 gene was inserted into pCA13 and got recombinant plasmid pCAp53.293 cells(a kind of adenovirus packing cells)was co transfected with pCAp53 and pBHG11,recombinant p53 adenovirus was obtained.Virus particle can be seen under electric microscopy and its diameter is about 80 nm.Virus titer is 6.7×10 8 PFU/mL.Recombinant p53 adenovirus have no influence on growth of normal lung cells,but it can cause death of bladder tumor cell (EJ)and primary culture breast cancer cell.MTT assay results showed that recombinant p53 adenovirus inhibited growth of p53 deficient tumor cell,such as bladder tumor cell(EJ)and epidermis cancer cell(A431)and inhibiting rate in 72 hours may be 86.7% and 78.4%.Flow cytometry test showed that recombinant p53 adenovirus induced apoptosis of tumor cell.Wild type p53 gene mediated by adenovirus vector may be used in the treatment for cancer.
出处
《生物工程学报》
CAS
CSCD
北大核心
1999年第4期516-521,共6页
Chinese Journal of Biotechnology
基金
国家自然科学基金高技术新探索项目
