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几种真菌诱导子对云南红豆杉细胞产生紫杉醇的影响 被引量:20

Effects of Fungus Elicitors on Taxol Production in Suspension Cells of Taxus yunnanensis
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摘要 The effects of the cultures of Penicilliun citrinum,Absidia glauca,Mucor rouxianus and Botrytis cinerea on the growth and taxol production in suspension cells of Taxus yunnanensis were investigated.The results showed that the addition of the cultures of Penicilliun citrinum or Botrytis cinerea into the suspension cell cultures of T.yunnanensis at the final stage of fast growing phase(the 12th day of culture) did not inhibited the cell growth,but dramatically increased taxol yield;The best result was obtained in the elicitation with Penicilliun citrinum (3.1 g/L dry mycelium) for 3 days,in which the taxol yield was increased by 1~2 times. The effects of the cultures of Penicilliun citrinum,Absidia glauca,Mucor rouxianus and Botrytis cinerea on the growth and taxol production in suspension cells of Taxus yunnanensis were investigated.The results showed that the addition of the cultures of Penicilliun citrinum or Botrytis cinerea into the suspension cell cultures of T.yunnanensis at the final stage of fast growing phase(the 12th day of culture) did not inhibited the cell growth,but dramatically increased taxol yield;The best result was obtained in the elicitation with Penicilliun citrinum (3.1 g/L dry mycelium) for 3 days,in which the taxol yield was increased by 1~2 times.
出处 《生物工程学报》 CAS CSCD 北大核心 1999年第4期522-524,共3页 Chinese Journal of Biotechnology
关键词 云南红豆杉 悬浮细胞培养 真菌诱导子 紫杉醇 Taxus yunnanensis , suspension cell culture, fungus elicitor, taxol
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参考文献3

  • 1陈永勤 朱蔚华.-[J].植物生理学通讯,1997,33:213-219.
  • 2Wu Y Q,Chromatgr & Related Technol,1997年,20卷,3147页
  • 3陈永勤,植物生理学通讯,1997年,33卷,213页

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