苏云金芽孢杆菌杀虫晶体蛋白基因导入大豆的研究

Studies on Transfer of Bt Gene into Glycine max

用苏云金芽孢杆菌(BacillusthuringiensisBerliner)杀虫晶体蛋白(Bt)基因和葡糖苷酸酶(GUS)基因通过基因枪轰击和根癌土壤杆菌(Agrobacteriumtumefaciens(SmithetTownsend)Conn)介导转入大豆(Glycinemax(L.)Merr.),诱导大豆转基因植株再生。大豆主栽品种“中黄4号”和品系8502未成熟子叶有较强体细胞胚分化能力。体细胞胚的脱水处理显著促进“中黄4号”体细胞胚的萌发。未成熟子叶的预培养有利于根癌土壤杆菌感染子叶外植体体细胞胚的分化。基因型和受体的选择,转基因体系的改进,体细胞胚的脱水处理等是提高大豆转基因效率的重要因素。

Transgenic plants were regenerated from soybean ( Glycine max (L.) Merr.) immature cotyledons bombarded by gene_gun with plasmid pGB14AB which contained Bacillus thuringiensis Berliner insecticidal crystal protein gene ( Bt gene )and GUS gene, and from cotyledon nodes infected with LBA4404 which contained pGB14AB. Immature cotyledons of “Zhonghuang No. 4” (variety) and 8502 (line) differentiated many more somatic embryos than others. Desiccation treatment of somatic embryos from immature cotyledons of “Zhonghuang No. 4” improved significantly their germination frequency. Preculturing of immature cotyledon was beneficial for somatic embryogenesis from the cotyledons infected with Agrobacterium tumefaciens (Smith et Townsend) Conn. High efficiency of transformation is related to selection of genotype and recipient, desiccation treatment of somatic embryos and improvement of transformed system in soybean.