摘要
通过根癌土壤杆菌介导法将外源抗虫基因导入4个陆地棉(GossypiumhirsutumL.)栽培品种。对转基因再生植株的移栽和嫁接研究发现,不同的定植方法对成活率和植株生长状态有显著的影响。直接移栽法、蛭石炼苗法和水培法的定植成活率均较低,缓苗时间也相对较长。而嫁接能够极大地提高再生植株的定植成活率并有效地缩短了缓苗时间,从而克服了棉花再生植株不易定植成活的困难,为基因工程乃至其他生物技术能够顺利地应用于棉花遗传改良奠定了基础。
Foreign insecticidal genes were transferred into 4 upland cotton ( Gossypium hirsutum L.) cultivars by Agrobacterium_ mediated transformation. It was found that planting style has a critical influence on the survival rate of the transgenic plantlets. Direct transplanting, culturing in liquid medium or vermiculite before transplanting were not proper for planting transgenic plantlets. The survival rate was quite low and hardening time was rather long. Graft could greatly enhance the survival rate and efficiently reduced the hardening time, thus paves the way for cotton improvements through genetic engineering and other biotechnology.
基金
"8 6 3"
"总理基金"
国家自然科学基金资助项目
关键词
陆地棉
转化
移栽
嫁接
转基因
Gossypium hirsutum, Transformation, Transplant, Graft
