摘要
棕色固氮菌(AzotobactervinelandiiLipmann)固氮酶MoFe蛋白经邻菲啉和空气处理后,成为部分缺失P_cluster和FeMoco的失活蛋白。与由Re2O7、高柠檬酸铁、Na2S和二硫苏糖醇(DTT)组成的无圆二色(CD)谱信号的重组液保温后,保温蛋白对乙炔和质子还原的活性都得以显著恢复;紫外和可见光CD谱虽有明显恢复,但仍与还原MoFe蛋白有所差异。这表明:1)保温的蛋白液中除含有未被邻菲啉等处理而破坏的完整MoFe蛋白外,还可能存在新组装的含Re的固氮酶;2)新组装的ReFe蛋白和MoFe蛋白可能在固氮能力上相似。
When the reduced MoFe protein from Azotobacter vinelandii Lipmann was treated with o _phenanthroline and air, an inactive protein partially deficient in both FeMoco and P_cluster could be obtained. After incubating the treated protein with a reconstituent solution containing Re 2O 7, ferric homocitrate, Na 2S and dithiothreitol, which had no circular dichroism (CD) signal, the ultraviolet and visible CD spectra, the C 2H 2 and H +_reduction activity of the incubated protein were significantly recovered. However, the spectra were somewhat different from those of the reduced MoFe protein. The results showed that: 1) in the incubated protein solution there was possibly a new recombined ReFe protein besides the intact MoFe protein which was not destroyed by the treatment with o _phenanthroline and air; 2) it might be possible that both ReFe protein and MoFe protein exhibited similar ability of nitrogen fixation, although they were somewhat different in structure.
基金
家自然科学基金资助项目
关键词
失活
钼铁蛋白
铼重组液
体外组装
含铼固氮酶
Inactive MoFe protein,Re_containing reconstituent solution,Re_containing nitrogenase reconstituted in vitro, Azotobacter vinelandii