摘要
研究了南主红豆杉(Taxuschinensisvar.maireiChengetFu)细胞双液相培养中前体饲喂和混合糖源作为生产培养基糖源对细胞生长和紫杉醇合成的影响。结果表明,前体饲喂(苯丙氨酸、苯甲酰胺和醋酸钠)或混合糖(麦芽糖和蔗糖)作为生产培养基糖源对南方红豆杉细胞单液相培养、更重要的是对其双液相培养的紫杉醇产量提高有显著促进作用,而且前体饲喂和混合糖源的协同作用对其双液相培养的紫杉醇产量提高有进一步的促进作用。与对照组(蔗糖作为糖源和无前体的单液相培养)相比,在南方红豆杉细胞双液相培养中,第10天加入前体,第11天加入混合糖源。
The effects of precursor feeding and mixed sugar supplements on the growth and taxol production of Taxus chinensis var. mairei Cheng et Fu in the biphasic_liquid culture were studied. Precursor feeding (phenylanine, benzamide and sodium acetate) and mixed sugars (maltose and sucrose) resulted in significant increase of taxol production not only in the monophasic_liquid culture but also in the biphasic_liquid culture of T. chinensis var. mairei . The synergistic function of precursor feeding on day 10 and mixed sugars on day 11 in the biphasic_liquid culture revealed more significant increase of taxol production, which was four times that of the control (the monophasic_liquid culture without precursors or mixed sugars).
基金
国家教委"跨世纪优秀人才培养计划"基金
天津市科委项目
关键词
紫杉醇
南方红豆杉
双液相培养
Taxol, Taxus chinensis var. mairei , Biphasic_liquid culture, Precursor feeding, Mixed sugar
