香石竹花瓣中乙烯诱导合成的 60kD蛋白功能的初步研究

A Preliminary Study on the Function of Ethylene Induced 60 kD Protein in Carnation Petal 

越来越多的研究表明植物器官的衰老与蛋白质合成密切相关[1～3]。乙烯在许多花卉衰老和一些水果的成熟过程中起重要作用[1,3],实验结果显示花对乙烯的反应依赖于新的蛋白质合成[2～5]。深入研究那些与衰老相关的蛋白质,特别是乙烯诱导合成的蛋白质的生化功能,对于了解掌握...

Antibody against the ethylene induced 60 kD protein (E60) in carnation (Dianthus caryophyllus L.) petal was obtained from immunized rabbit serum. The E60 was purified with gel filtration, DEAE_Sephadex and Hydroxylapatite column. By combining IEF, SDS_PAGE and immune_blot analysis, the ethylene induced 60 kD protein was found to be an pI 6.3 peroxidase.