降低差异显示PCR假阳性和提高重复性的几种策略

Several Strategies to Reduce False Positive of DD PCR and Improve Its Reproducibility.

简要介绍了几种提高ｍＲＮＡ差异显示重复性和降低假阳性的策略． ｍＲＮＡ 差异显示技术从建立时起就引起了科学家的广泛兴趣， 是克隆不同生理或病理过程中差异表达基因的一种高灵敏度、简单、有效的方法． 但ｍＲＮＡ差异显示因其假阳性率高和重复性低，

Differential display PCR(DD PCR)has attracted widespread interest since it was established. It is a sensitive, simple and powerful method to clone differentially expressed gene fragment in different courses of physiology and pathology. But the main drawback of differential display is high false positive and low reproducibility. This restricted its application in life science. Several strategies to reduce false positive of differential display and improve its reproducibilty was reviewed briefly.